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goat anti green fluorescent protein  (Novus Biologicals)


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    Structured Review

    Novus Biologicals goat anti green fluorescent protein
    Goat Anti Green Fluorescent Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+gfp/GFP+Antibody/bio_rxiv__64898__2026__04__17__719280-155-18-25
    Average 96 stars, based on 132 article reviews
    goat anti green fluorescent protein - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Magel2 in hypothalamic POMC neurons influences the impact of stress on anxiety-like behavior and spatial learning associated with a food reward in male mice
    Article Snippet: .. The sections were incubated with 0.3% Triton X-100 at room temperature (RT) for 30 min, and then blocked in PBS buffer containing 5% donkey serum, 2% bovine serum albumin and 0.15% Triton X-100 for 1 h at RT and then incubated with goat anti-GFP (NB100-1770, Novus), rabbit anti-POMC (1:1000, H-029-30, Phoenix biotech), and rabbit anti-MAGEL2 [1:2000, a gift from Dr. Tacer ( )] antibodies for overnight at a cold room, and then sections were washed three times in PBS and incubated with Alexa 488 anti-goat IgG for GFP (1:1000, 705–545-147, Jackson ImmunoResearch), Alexa 594 anti-rabbit IgG for POMC and MAGEL2 (1:1000, 711-585-152, Jackson ImmunoResearch) for 3 h at RT. .. After washing, the sections were stained with DAPI and mounted using VECTASHIELD medium (H-2000, Vector Lab.).

    Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception.
    Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/ 36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..

    Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception
    Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..

    Blocking Assay:

    Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception.
    Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/ 36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..

    Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception
    Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..

    other:

    Article Title: Med13 is involved in the radial migration and contralateral projection of cortical neurons via PlxnA4.
    Article Snippet: The following primary antibodies were used: goat anti-GFP (1:2000; NB100-1770, Novus Biologicals), rabbit anti-GFP (1:1000; A11122, Invitrogen), rabbit anti-PlxnA4 (1:200; 3816S, Cell Signaling Technology), guinea pig anti-NeuN (1:300; OBPGP006, Oasis), rabbit anti-Cux1 (1:200; OB-PRB038-01, Oasis), rabbit anti-Satb2 (1:500; ab92446, Abcam), guinea-pig anti-Ctip2 (1:300; OB-PGP012-01, Oasis), guinea pig anti-Tle4 (1:300; OB-PGP086-01, Oasis), rabbit anti-Blbp (1:200; ab32423, Abcam).



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    Identification of transduced RGCs using in vivo fluorescence fundoscopy and confocal imaging (A–O) Fluorescence fundus images are shown from two example rats. The schematic for rat one illustrates the left eye at post-operative days (PODs) (A) 5, (C) 9, (E) 13, (G) 17, (I) 21, and (K) 27. Red lines depict the intraocular vasculature used to align fundus images across days. Insets (B, D, F, H, J, and L) show magnified views of regions with prominent labeling, as indicated by the dashed white rectangles (A, C, E, G, I, and K, respectively). Examples of transduced RGCs are indicated with Roman numerals (i–xii), several of which are present across multiple imaging time points. The schematic for rat two (M–O) illustrates an example of expression longevity starting from POD 14 through POD 146. Note: variations in overall fluorescence intensity across time points are likely caused by differences in fundus camera positioning between imaging sessions; we therefore do not attribute these changes to fluctuations in viral expression. The spatial pattern of labeled RGCs remains stable, indicating persistent expression. (P) Injection of AAV2retro-CAG-ReaChR-mCitrine into the left SC resulted in labeling across tectal layers, as indicated by the arrows. (Q) This injection resulted in fluorescence fundoscopic detection of mCitrine-positive RGC labeling within the nasal quadrant of the right eye. (R) A low-magnification confocal photomicrograph of the eyecup <t>following</t> <t>anti-GFP</t> immunofluorescent amplification. The dashed box delineates the region shown in (S). (S) A confocal photomicrograph shown at the estimated scale as the fluorescence fundus image in (Q). Alignment of these images was achieved using the major blood vessels. Roman numerals i and ii identify examples of vessel bifurcation used to match the images from fluorescence fundoscopy with those from confocal imaging. (T–W) Examples of retrogradely transduced RGCs are depicted with arrows in aligned fluorescence fundoscopy images (T and V) and corresponding confocal images (U and W). Solid white arrows mark RGCs clearly visible in both imaging modalities. A hollow arrow in (V)/(W) marks an RGC that is evident in the confocal image (W) but not clearly distinguishable in the fundus image (V), highlighting the potential bias of fluorescence fundus imaging toward more brightly labeled cells. .
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    Identification of transduced RGCs using in vivo fluorescence fundoscopy and confocal imaging (A–O) Fluorescence fundus images are shown from two example rats. The schematic for rat one illustrates the left eye at post-operative days (PODs) (A) 5, (C) 9, (E) 13, (G) 17, (I) 21, and (K) 27. Red lines depict the intraocular vasculature used to align fundus images across days. Insets (B, D, F, H, J, and L) show magnified views of regions with prominent labeling, as indicated by the dashed white rectangles (A, C, E, G, I, and K, respectively). Examples of transduced RGCs are indicated with Roman numerals (i–xii), several of which are present across multiple imaging time points. The schematic for rat two (M–O) illustrates an example of expression longevity starting from POD 14 through POD 146. Note: variations in overall fluorescence intensity across time points are likely caused by differences in fundus camera positioning between imaging sessions; we therefore do not attribute these changes to fluctuations in viral expression. The spatial pattern of labeled RGCs remains stable, indicating persistent expression. (P) Injection of AAV2retro-CAG-ReaChR-mCitrine into the left SC resulted in labeling across tectal layers, as indicated by the arrows. (Q) This injection resulted in fluorescence fundoscopic detection of mCitrine-positive RGC labeling within the nasal quadrant of the right eye. (R) A low-magnification confocal photomicrograph of the eyecup <t>following</t> <t>anti-GFP</t> immunofluorescent amplification. The dashed box delineates the region shown in (S). (S) A confocal photomicrograph shown at the estimated scale as the fluorescence fundus image in (Q). Alignment of these images was achieved using the major blood vessels. Roman numerals i and ii identify examples of vessel bifurcation used to match the images from fluorescence fundoscopy with those from confocal imaging. (T–W) Examples of retrogradely transduced RGCs are depicted with arrows in aligned fluorescence fundoscopy images (T and V) and corresponding confocal images (U and W). Solid white arrows mark RGCs clearly visible in both imaging modalities. A hollow arrow in (V)/(W) marks an RGC that is evident in the confocal image (W) but not clearly distinguishable in the fundus image (V), highlighting the potential bias of fluorescence fundus imaging toward more brightly labeled cells. .
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    Image Search Results


    Identification of transduced RGCs using in vivo fluorescence fundoscopy and confocal imaging (A–O) Fluorescence fundus images are shown from two example rats. The schematic for rat one illustrates the left eye at post-operative days (PODs) (A) 5, (C) 9, (E) 13, (G) 17, (I) 21, and (K) 27. Red lines depict the intraocular vasculature used to align fundus images across days. Insets (B, D, F, H, J, and L) show magnified views of regions with prominent labeling, as indicated by the dashed white rectangles (A, C, E, G, I, and K, respectively). Examples of transduced RGCs are indicated with Roman numerals (i–xii), several of which are present across multiple imaging time points. The schematic for rat two (M–O) illustrates an example of expression longevity starting from POD 14 through POD 146. Note: variations in overall fluorescence intensity across time points are likely caused by differences in fundus camera positioning between imaging sessions; we therefore do not attribute these changes to fluctuations in viral expression. The spatial pattern of labeled RGCs remains stable, indicating persistent expression. (P) Injection of AAV2retro-CAG-ReaChR-mCitrine into the left SC resulted in labeling across tectal layers, as indicated by the arrows. (Q) This injection resulted in fluorescence fundoscopic detection of mCitrine-positive RGC labeling within the nasal quadrant of the right eye. (R) A low-magnification confocal photomicrograph of the eyecup following anti-GFP immunofluorescent amplification. The dashed box delineates the region shown in (S). (S) A confocal photomicrograph shown at the estimated scale as the fluorescence fundus image in (Q). Alignment of these images was achieved using the major blood vessels. Roman numerals i and ii identify examples of vessel bifurcation used to match the images from fluorescence fundoscopy with those from confocal imaging. (T–W) Examples of retrogradely transduced RGCs are depicted with arrows in aligned fluorescence fundoscopy images (T and V) and corresponding confocal images (U and W). Solid white arrows mark RGCs clearly visible in both imaging modalities. A hollow arrow in (V)/(W) marks an RGC that is evident in the confocal image (W) but not clearly distinguishable in the fundus image (V), highlighting the potential bias of fluorescence fundus imaging toward more brightly labeled cells. .

    Journal: Cell Reports Methods

    Article Title: Projection targeting with phototagging to study the structure and function of retinal ganglion cells

    doi: 10.1016/j.crmeth.2026.101308

    Figure Lengend Snippet: Identification of transduced RGCs using in vivo fluorescence fundoscopy and confocal imaging (A–O) Fluorescence fundus images are shown from two example rats. The schematic for rat one illustrates the left eye at post-operative days (PODs) (A) 5, (C) 9, (E) 13, (G) 17, (I) 21, and (K) 27. Red lines depict the intraocular vasculature used to align fundus images across days. Insets (B, D, F, H, J, and L) show magnified views of regions with prominent labeling, as indicated by the dashed white rectangles (A, C, E, G, I, and K, respectively). Examples of transduced RGCs are indicated with Roman numerals (i–xii), several of which are present across multiple imaging time points. The schematic for rat two (M–O) illustrates an example of expression longevity starting from POD 14 through POD 146. Note: variations in overall fluorescence intensity across time points are likely caused by differences in fundus camera positioning between imaging sessions; we therefore do not attribute these changes to fluctuations in viral expression. The spatial pattern of labeled RGCs remains stable, indicating persistent expression. (P) Injection of AAV2retro-CAG-ReaChR-mCitrine into the left SC resulted in labeling across tectal layers, as indicated by the arrows. (Q) This injection resulted in fluorescence fundoscopic detection of mCitrine-positive RGC labeling within the nasal quadrant of the right eye. (R) A low-magnification confocal photomicrograph of the eyecup following anti-GFP immunofluorescent amplification. The dashed box delineates the region shown in (S). (S) A confocal photomicrograph shown at the estimated scale as the fluorescence fundus image in (Q). Alignment of these images was achieved using the major blood vessels. Roman numerals i and ii identify examples of vessel bifurcation used to match the images from fluorescence fundoscopy with those from confocal imaging. (T–W) Examples of retrogradely transduced RGCs are depicted with arrows in aligned fluorescence fundoscopy images (T and V) and corresponding confocal images (U and W). Solid white arrows mark RGCs clearly visible in both imaging modalities. A hollow arrow in (V)/(W) marks an RGC that is evident in the confocal image (W) but not clearly distinguishable in the fundus image (V), highlighting the potential bias of fluorescence fundus imaging toward more brightly labeled cells. .

    Article Snippet: Goat Anti-GFP , Rockland , Cat# 600-101-215; RRID: AB_218182.

    Techniques: In Vivo, Fluorescence, Imaging, Labeling, Expressing, Injection, Amplification