Incubation:Article Title: Magel2 in hypothalamic POMC neurons influences the impact of stress on anxiety-like behavior and spatial learning associated with a food reward in male mice
Article Snippet: .. The sections were incubated with 0.3% Triton X-100 at room temperature (RT) for 30 min, and then blocked in PBS buffer containing 5% donkey serum, 2% bovine serum albumin and 0.15% Triton X-100 for 1 h at RT and then incubated with goat anti-GFP (NB100-1770, Novus), rabbit anti-POMC (1:1000, H-029-30, Phoenix biotech), and rabbit anti-MAGEL2 [1:2000, a gift from Dr. Tacer ( )] antibodies for overnight at a cold room, and then sections were washed three times in PBS and incubated with Alexa 488 anti-goat IgG for GFP (1:1000, 705–545-147, Jackson ImmunoResearch), Alexa 594 anti-rabbit IgG for POMC and MAGEL2 (1:1000, 711-585-152, Jackson ImmunoResearch) for 3 h at RT. .. After washing, the sections were stained with DAPI and mounted using VECTASHIELD medium (H-2000, Vector Lab.).
Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception.
Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/ 36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..
Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception
Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..
Blocking Assay:Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception.
Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/ 36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..
Article Title: The axon initial segment-associated microglia regulate neuronal activity and visual perception
Article Snippet: .. The recorded brain slices were incubated in a blocking solution (10% normal goat serum and 0.1% Triton X-100 in PBS) for ~2 h at room temperature, and then incubated with primary antibodies, including goat anti-GFP (Novus, NB100-1770, 1:1000), mouse anti-AnkG (NeuroMab, N106/36, 1:500), mouse anti-Beta4-spectrin (NeuroMab, 75-377, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:1000), rabbit anti-NeuN (Cell Signaling Technology, 24307S, 1:500), rabbit anti-THIK-1 (Alomone, APC-121, 1:500), rabbit anti-Caspase3 (Cell Signaling Technology, 9661 T, 1:500), mouse anti-Gephyrin (SYSY, 417011, 1:500), rabbit anti-vGAT (SYSY, 131003, 1:500), rabbit anti-Homer1 (Abcam, ab184955, 1:500), mouse anti-vGlut2 (Abcam, ab79157, 1:500), rat anti-MBP (Abcam, ab7349, 1:500), pig anti-Oligo2 (Millipore, ABE1024, 1:500), rabbit anti-ITGB1 (Proteintech, 12594-1-AP, 1:500) and rabbit anti-NALCN (Alomone, ASC-022, 1:500) for 24 h at 4 °C. ..
other:Article Title: Med13 is involved in the radial migration and contralateral projection of cortical neurons via PlxnA4.
Article Snippet: The following primary antibodies were used: goat anti-GFP (1:2000; NB100-1770, Novus Biologicals), rabbit anti-GFP (1:1000; A11122, Invitrogen), rabbit anti-PlxnA4 (1:200; 3816S, Cell Signaling Technology), guinea pig anti-NeuN (1:300; OBPGP006, Oasis), rabbit anti-Cux1 (1:200; OB-PRB038-01, Oasis), rabbit anti-Satb2 (1:500; ab92446, Abcam), guinea-pig anti-Ctip2 (1:300; OB-PGP012-01, Oasis), guinea pig anti-Tle4 (1:300; OB-PGP086-01, Oasis), rabbit anti-Blbp (1:200; ab32423, Abcam).
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